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Optimized protocol and validation studies using amplificate dilution series
| Target | Annealing temp (°C)/time (sec) | Extension temp (°C)/time (sec) | ∆Ct (RT+ to RT−) | slope | Correlation-Coefficient (Pearson) R2 | Verified dynamic range |
|---|---|---|---|---|---|---|
| XBP-1 | 65/30 | 72/20 | not detected | −3.537 | 0.997 | 105 |
