Figure 2.

Hepcidin mRNA expression in the liver. cDNA synthesized from liver RNA of transgenic mice, lacking the expression of either glutathione peroxidase-1 (gpx-1^−/−) or catalase (catalase^−/−), and wild-type mice, fed with plain water (H₂O) or ethanol (alc.), was employed to determine hepcidin mRNA expression by Taqman real-time PCR. (A) Hepcidin gene expression in water or alcohol-fed transgenic mice and alcohol-fed wild-type mice was expressed as-fold hepcidin expression of that in wild-type mice fed with water. (B) Hepcidin gene expression in alcohol-fed gpx-1^−/− mice was expressed as-fold hepcidin expression of that in gpx-1^−/− mice fed with water. Asterisks indicate statistical significance (p < 0.05).