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Liver ATF4 and ATF6 mRNA expression. cDNA synthesized from liver RNA of wild-type and catalase−/− or gpx-1−/− transgenic mice, fed with plain water (H2O) or ethanol (alc.), was employed to determine ATF4 (A) and ATF6 (B) mRNA expression by real-time PCR. Gene expression levels in alcohol fed wild-type and untreated or alcohol-fed transgenic mice were expressed as-fold ATF expression of that in wild-type mice fed with water. Asterisks indicate statistical significance (p < 0.05).
