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. 2015 Jun 11;5(2):1178–1194. doi: 10.3390/biom5021178

Figure 2.

Figure 2

(A) Inhibition of L. monocytogenes 39-2 by overlay of prior spotted and incubated culture spots (“deferred antagonism”). Spotted cultures, clockwise from top: Lb. delbrueckii 4797-2 (Bac− control), Lb. curvatus FS47, En. faecium FS56-1, Pediococcus acidilactici Bac3, Lactococcus lactis FS91-1, En. thailandicus FS92, En. faecium FS97-2; (B) Inhibition of L. monocytogenes indicator lawn spotted with non-neutralized cell free spent broth from Bac+ (Lb. curvatus FS47) and Bac− (Lb. delbrueckii 4797-2) strains (“concurrent antagonism”). The presence of “spontaneous resistant” indicator colonies can be clearly observed in the curvaticin FS47 inhibition zone.