Fig. 2.

α7 and Gprin1 associate in the growth cone. (a) Hippocampal neurons from P0 pups was cultured for 3 DIV. Neurons were probed with fBgtx (green) and anti-Gprin1 Abs (red) (top images). A heat-map measure of the co-signal (bottom image) shows the distribution and co-localization of the two proteins in the growing axon and GC. Colocalization was highest in the soma, growth cone (GC), and branch points (BP) (arrows). (b) Localization of the fBgtx and Gprin1 signals in the GC. CZ: central zone. (c) Protein detection of α7 and Gprin1 within GC fraction (GCF) obtained from P0 pups as described in Materials and Methods. GAP-43 is used as a marker for the GCF. Cell fraction (CF). (d) An anti-α7 and anti-Gprin1 Ab was used to IP α7 and Gprin1 proteins from the GCF. Western blot detection confirms interaction of the two proteins. Protein identity was also determined by in-gel digest mass spectrometry (Table S2). IgG was used as an antibody control.