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. 2015 Jul 10;8:12. doi: 10.1186/s13069-015-0030-0

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© Nakasatomi et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Localization of BrdU-positive cells in the normal rat kidneys after long-term BrdU labeling. a–c BrdU was given intraperitoneally to normal rats for 4 weeks. Double staining of BrdU with several tubular (a), glomerular (b), and interstitial (c) markers was performed. Markers: aquaporin-1 (AQP-1), lotus tetragonolobus agglutinin (LTA), Tamm-Horsfall glycoprotein (THP), aquaporin-2 (AQP-2), alpha-SMA, vimentin, CD31, WT1, and aminopeptidase P. DAPI (blue). Magnification, ×1000. d Quantitative analysis of percentage of BrdU-positive cells per total marker-positive cells. Values are means ± SE (n = 5)