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. 2015 Jul 10;8:12. doi: 10.1186/s13069-015-0030-0

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© Nakasatomi et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Phenotypes of BrdU-positive tubular cells in the UUO kidneys. a Expression of myofibroblast-related markers in kidneys at 7 days after UUO. BrdU (red), markers (green), and DAPI (blue). Magnification, ×1000. Arrowheads indicate BrdU-positive cells coexpressing myofibroblast markers. b Number of alpha-SMA-positive cells and alpha-SMA/BrdU double-positive cells in the interstitium of contralateral and UUO kidneys at 8 days after operation. Values are means ± SE (n = 5). c Expression of AQP-1 in kidneys at 7 days after UUO. Arrowheads indicate BrdU-positive cells losing AQP-1 expression. BrdU (red), AQP-1 (green), and DAPI (blue). Magnification, ×1000. d Expression of inflammatory cell markers in kidneys at 7 days after UUO. DAPI (blue). Magnification, ×1000