Fig. 4.

The quantities of C57BL/6J mouse hematopoietic precursors from cBM and eBM. Flow cytometric analysis of cBM and eBM was used to identify precursor populations using the gates indicated (a). Total cells were defined as live cells – lacking PI staining – from which doublets were excluded by electronic gating (not shown). The median frequencies (b) and numbers (c) of cells recovered of each precursor population are shown (n = 5). Note, that these data are shown on a logarithmic scale. The effects of needle size on the recovery of cBM (d) and eBM (e) cells are shown for 6 samples for each group