Figure 4.

β-adrenergic receptor expression and responsiveness to isoproterenol in differentiated facial and abdominal preadipocytes. On day 14, cultures remained untreated (CDR) or were incubated with 10 μM isoproterenol (CDR+ IPTL) for up to one week. (A) Acute glycerol release after 5 hour isoproterenol treatment (2 μM). Data represented as mean ± SEM. Abdominal cells, n = 5 donors and facial cells, n = 7 donors. *Student t test P < 0.05. (B) Representative image of facial and abdominal adipocytes before and after 1 week of isoproterenol exposure. (C) After 1 week of isoproterenol treatment, the expression of lipid metabolic genes was evaluated by qPCR analysis and was normalized by expression of the RPL13A gene. Abdominal cells, n = 6 donors and facial cells, n = 4 donors. CD, control differentiation; CDR, CD + 10 μM rosiglitazone; ADRB2, β2-adrenergic receptor. Passages 2 until 6 were used for abdominal cells and passages 3 untill 11 were used for facial cells.