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Assembly of a MAGP1-rich extracellular matrix by adipose tissue stromal cells. The stromal-vascular cell fraction of subcutaneous white adipose tissue from wild-type mice was cultured in growth media (DMEM/F12 media supplemented with 10% FBS and antibiotics) for 7 d Cells were fixed using cold methanol. MAGP1 was visualized using a primary antibody targeting murine MAGP1 and Alexa Fluor 555-conjugated secondary antibody (green). Nuclei were visualized using Hoechst nuclear dye (blue).
