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. 2014 Dec 17;4(2):113–122. doi: 10.4161/21623945.2014.973772

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Figure 2. — Growth hormone regulates ZAG and inhibits lipid accumulation in 3T3-L1 adipocytes in vitro. (A) The effect of 6-day GH treatment on ZAG gene expression in differentiated 3T3-L1 and primary human adipocytes. (B) The effect of 6-day GH treatment on lipid accumulation and (C) expression of FAS, ACC1, DGAT mRNA in differentiated 3T3-L1 adipocytes. (D) Association of ZAG gene expression with DGAT mRNA. Effect of 6-day GH treatment on (E) basal and (F) isoproterenol-induced lipolysis in differentiated 3T3-L1 adipocytes. Presented data represent an average value of 3 (A, B) or 4 (C, D, E, F) independent experiments. ACC1, acetyl CoA carboxylase 1; DGAT, acyl CoA:diacylglycerol acyltransferase; FAS, fatty acid synthase; GH, growth hormone; ZAG, zinc-α2-glycoprotein; *P < 0.05; **P < 0.01; ***P < 0.001.