Figure 3.

Silencing ZAG eliminates GH effects on lipid metabolism in vitro. The effect of ZAG silencing on (A) ZAG gene and protein expression in differentiated human primary adipocytes. Representative western blot shows ZAG protein in siRNA transfected adipocytes when compared to control - scramble transfected cells. The effect of 6-day growth hormone treatment and transfection of ZAG/scramble siRNA on (B, C) lipid accumulation, (D) basal lipolysis (glycerol release in absence of insulin) and (E) insulin sensitivity (glycerol release in presence of 66 nM insulin) in differentiated human primary adipocytes. The results represent an average of 3 (A, B, C, D) or 4 (E) independent experiments. GH, growth hormone; ZAG, zinc-α2-glycoprotein; TG, triglycerides; picture presented in (C) was taken at magnification of 50x; *P < 0.05; **P < 0.01; ***P < 0.001.