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Alkaline phosphatase (ALP) activity and staining. (A) ALP activity of E.BMP-2 with growth factors treatment at 3, 7 and 14 days in osteogenic differentiation. Results are presented as mean ± standard error of the mean (SEM); *, **, p < 0.05. (B) ALP staining at 3, 7 and 14 days for osteogenesis; magnification = ×10
