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Alizarin red-S (AR-S) staining and calcium concentration. (A) AR-S staining at 7, 14 and 21 days of osteogenesis; magnification = ×10. (B) Calcium concentrations at 7, 14 and 21 days in osteogenic differentiation; the results are presented as mean ± SEM; *, **, p < 0.05
