Figure 5.

Gene expression in hMSCs by RT–PCR. (A) Runx-2 expression was increased at 7 days of treatment. Especially the groups treated in combination with EGF or FGF showed increased expression up to 14 days. (B) The expression of BSP was markedly elevated with time in cells treated in combination with EGF, FGF or PDGF. EGF and FGF increased E.BMP2-induced osteopontin expression in a time-dependent manner. OPN expression was higher in the combination treatment group with EGF or FGF compared to the other groups; C, control; I, induction of osteogenesis; B, E.BMP-2; E, EGF; F, FGF; P, PDGF; V, VEGF