Table 1.

Gene-specific primers for RT–PCR analysis

Genes	Sequence (5'→3')	Annealing temperature (°C)	Prod size (bp)
ALP	F: TGGAGCTTCAGAAGCTCAACACCA	51	453
	R: ATCTCGTTGTCTGAGTACCAGTCC
Runx-2	F: CCGCACGACAACCGCACCAT	57	530
	R: CGCTCCGGCCCACAAATCTC
Osteopontin	F: CCAAGTAAGTCCAACGAAAG	55	348
	R: GGTGATGTCCTCGTCTGTA
BSP	F: CGAAGACAACAACCTCTCCAAATG	51	257
	R: ACCATCATAGCCATCGTAGCCTTG
Collagen I	F: GGTGTAAGCGGTGGTGGTTAT	57	335
	R: GCTGGGATGTTTTCAGGTTGG
GAPDH	F: CCAGAACATCATCCCTGCCTCTAC	54	554
	R: GGTCTCTCTCTTCCTCTTGTGC