Fig. 4.

SLC34A2 suppressed tumor growth and lung metastasis of NSCLC in vivo. a The expressions of SLC34A2 in A549-P-S, A549-P and A549 cells were confirmed by Western blot. b The concentrations of phosphorous in the supernatant of medium were measured by using a phosphorous measurement kit in A549-P-S, A549-P and A549 cells. c Tumor growth curve (each group contained 8 mice). d Tumor wet weight. Mice were killed for measurement of tumor weight at 60 days after inoculation. e Lungs from mice injected with A549-P-S, A549-P and A549 cells were injected intratracheally with India ink and fixed in AAF solution. f The numbers of lung nodules were counted under a dissecting microscope