Table 1.
Patient clinical features and SLC34A2 expression profile
| Patient No. | Age | Gender | Histological grade | Clinical stage | Normalised SLC34A2 amount in tumour tissue relative to adjacent normal tissue 2-ΔΔCt | Lymph node metastatic or non-metastatic |
|---|---|---|---|---|---|---|
| 1 | 54 | F | A | IIIA | 0.5675 | Yes |
| 2 | 53 | F | A | IA | 0.0715 | No |
| 3 | 74 | F | A | IIA | 0.0585 | Yes |
| 4 | 71 | M | A | IB | 0.1675 | No |
| 5 | 52 | F | A | IB | 0.2573 | No |
| 6 | 59 | F | A | IIIB | 0.2829 | Yes |
| 7 | 79 | F | A | IB | 0.4157 | No |
| 8 | 74 | F | A | IB | 0.3364 | No |
| 9 | 61 | F | A | IIIA | 0.1702 | Yes |
| 10 | 69 | F | A | IIB | 0.4994 | No |
| 11 | 64 | F | A | IIA | 3.3600 | No |
| 12 | 67 | M | A | IIA | 0.6622 | No |
| 13 | 81 | M | S | IV | 0.2813 | Yes |
| 14 | 50 | M | S | IIA | 0.1462 | No |
| 15 | 55 | M | S | IIIA | 0.5016 | Yes |
a. M male, F female, A adenocarcinoma, S squamous cell carcinoma. b. Relative quantification was performed by the 2-ΔΔCt method with the adjacent normal lung tissue sample as a calibrator. Data show the means from three independent analyses. Every independent analysis was carried out after the RNA extraction step. Total RNA was poly-A tailed, reverse transcripted, and then real-time PCR tested. ΔCT obtained from real-time PCR was subject to paired t-test (ΔCT = CTSLC34A2-CTβ-actin). The expression levels of SLC34A2 in tumor tissues were significantly lower than adjacent normal tissues (P < 0.05)