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. 2015 Jul 10;16(1):517. doi: 10.1186/s12864-015-1728-5

Table 4.

Comparison of fold change values from RNA-seq data and qRT-PCR in 2 h LPS and Poly I:C treated BV-2 microglia cells

RNA-seq fold change qRT-PCR fold change
Gene symbol Gene accession ID LPS_2h Poly I:C_2h LPS_2h Poly I:C_2h
Il-1ß NM_008361 93.82 96.13 89.02 96.72
Il1A NM_010554 57.04 82.69 67.98 99.02
TNF NM_001278601 51.12 45.94 55.94 69.01
PTGS2 NM_011198 32.41 51.93 36.5 35.09
CXCL10 NM_021274 34.74 88.57 12.53 87.45
CCL4 NM_013652 50.02 60.01 65.23 42.25
CCL7 NM_013654 17.41 44.98 52.23 35.26
CCL3 NM_011337 37.15 22.61 39.66 22.23
NFκBIA NM_010907 38.57 33.60 42.36 30.21
IRF7 NM_001252600 21.59 34.28 19.68 26.35
JUNB NM_008416 20.3 22.01 22.25 22.02
IRF1 NM_001159393 14.31 26.35 16.79 18.23
CLEC4E NM_019948 53.91 53.29 47.52 45.25
GPR84 NM_030720 44.69 47.16 51.25 41.23
SLC15A3 NM_023044 18.0 16.23 22.03 11.23
KDM4A NM_001161823 23.29 18.41 19.55 16.23
KLF7 NM_033563 4.89 3.85 1.04 0.909
IRAK3 NM_028679 4.97 4.25 0.77 0.89