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. 2015 Apr 16;56(4):669–677. doi: 10.1093/jrr/rrv023

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© The Author 2015. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 2. — Suppression of DMPO-OH generation by hangeshashinto (HST) for several •OH sources. (A) Suppression of X-ray–induced DMPO-OH by HST. Reaction mixtures containing 30-mM DMPO and various concentrations of HST were irradiated with 32 Gy X-rays. X-band EPR measurements were conducted 5 min after X-ray irradiation. (B) Suppression of UV + H₂O₂ induced DMPO-OH by HST. Reaction mixtures containing 30-mM DMPO, 1-mM H₂O₂ and various concentrations of HST were irradiated with UV light. The time course of DMPO-OH generation during UV irradiation was measured. (C) Suppression of the Fenton reaction–induced DMPO-OH by HST. Reaction mixtures containing 30-mM DMPO, 1-mM H₂O₂, 1-mM FeSO₄ and various concentrations of HST were prepared and measured by X-band EPR. The columns/marks and error bars indicate the mean ± standard deviation based on three samples. *, ** and ^## indicates significance compared with the control as P < 0.05, P < 0.01 and P < 0.001, respectively.