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. 2015 Jun 11;56(4):700–708. doi: 10.1093/jrr/rrv026

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© The Author 2015. Published by Oxford University Press on behalf of The Japan Radiation Research Society and Japanese Society for Radiation Oncology.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.

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Fig. 4. — Expression levels of inflammatory and anti-inflammatory cytokines in peripheral blood and liver tissue. Samples were collected 6 h after liver irradiation. Enzyme-linked immunosorbent assay (ELISA) (A) and Real-Time quantitative Reverse Transcription-PCR (RT-qPCR) (B) analyses showed that TNF-α, IL-β and IL-6 were all significantly increased after RT, and these effects were partially decreased by MSC-CM administration. Expression of the anti-inflammatory cytokine IL-10 was significantly increased by MSC-CM treatment. Data are shown as means ± SD. **P < 0.01 (DMEM group versus control group). ^##P < 0.01 and ^#P < 0.05 (MSC-CM group versus DMEM group).