Figure 4.

Reduced expression of IFN-γ and CXCR3 ligands in CXCR3-deficient mice following bleomycin-induced lung injury. Levels of IFN-γ protein in BAL (A) and in lung homogenates (B) in CXCR3-deficient and WT mice following bleomycin-induced lung injury were measured using ELISA. *P < 0.01; **P < 0.05. (C) Levels of CXCR3 ligand CXCL10 mRNA in the mouse lungs treated with bleomycin intratracheally at indicated days were assayed by Northern blot. Aldolase was used as an internal control for equal loading of samples. k, CXCR3^–/–. (D) Levels of CXCR3 ligand CXCL10 protein in the mouse lungs treated with bleomycin intratracheally at indicated days were assayed with ELISA. Similar results were obtained in three separate experiments. *P < 0.01; **P < 0.05.