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. 2015 Jun 20;15:63. doi: 10.1186/s12935-015-0215-z

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© Zeng et al. 2015

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Expression of Six1 in prostate cancer tissues and normal prostate tissues detected by western blotting and immunochemistry. a Relative mRNA expression of Six1 normalized to β-actin was calculated (n = 8), ^★ indicates P < 0.05. b Expressions of Six1 protein in 8 paired tissues were examined by western blotting. Micrographs showed weak (c, c), moderate (d, d), and strong (e, e) staining of Six1 in prostate cancer tissues, as well as low (f, f) expression of Six1 in normal prostate tissues (upper magnification × 100, lower panel: magnification × 400.)