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Levels of cyclin D1, CDK4, p53 and p21 protein expression in A375 derived cell lines. A. Cyclin D1 and CDK4 protein expression in the indicated cell lines was determined by Western blotting. β-actin levels are shown for reference. B, C. Quantification of the blots in A. D. p53 and p21 protein expression in the indicated cell lines determined by Western blotting. β-actin is shown as a reference. E, F. Quantification of the blots shown in D. *P < 0.05, ***P < 0.001.
