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Primer sequence, length, and PCR conditions for methylation analysis
| Gene | Primer* | Sequence (5’→3’) | Length | Ta# (°C) | Amplified Length (bp) |
|---|---|---|---|---|---|
| Jagged1 | tag-FW | aggaagagagAAAATTTTTTTGGAGTTAGGTTTGT | 10+25 | 56 | 314 |
| T7-RV | cagtaatacgactcactatagggagaaggctAACCCATCTCTTACCACCCAA | 31+21 | |||
| Notch1 | tag-FW | aggaagagagTTTTTTTAGGGGTTATTGAAGTTGA | 10+25 | 56 | 282 |
| T7-RV | cagtaatacgactcactatagggagaaggctCCCCTACTACAACCAAAAAACCTAT | 31+25 |
FW, forward; RV, reverse.
Ta, annealing temperature.
