Figure 7.

Snail interacts with Id2. A. OSCC cells were treated with or without TNF-α (10 ng/ml) for 72 h, then cells were immunoprecipitated with anti-Snail antibodies and control IgG and analyzed by Western blotting with the indicated anti-Snail and anti-Id2 antibodies. B and C. Id2 knockdown prevent Snail expression in SCC9 cells after incubation with TNF-α. SCC9 cells after dealing with TNF-α were transfected with either control siRNA (siNC) or Id2 siRNA (siId2). Cells were analyzed for Id2, Snail and epithelial and mesenchymal markers by real-time RT-PCR and western blot. GAPDH was used as a control. Each bar represents the mean ± s.d. *P<0.05. β-actin was employed as a loading control.