Figure 3.

A. Steatohepatitis was detected in hepatic tissues from the DEN-OVE26 mice by H & E staining. The histology of steatohepatitis characterized by inflammation and hepatocellular ballooning. The arrows indicate Mallory- Denk bodies within ballooned hepatocytes. B. TG significantly increased in the OVE26 mice compared to FVB mice. The body weights were decreased in the OVE26 mice compared to FVB mice. Liver weights were significantly increased in the OVE26 mice compared to FVB mice. C. FGF21 protein levels were significantly decreased in the diabetic OVE26 mice treated with DEN, compared to the untreated OVE26 mice. D. High glucose induced FGF21 mRNA expression in both hepatocytes and hepatoma cells in vitro. However, FGF21 expression of hepatoma cells decreased after 12 hours and went back to the baseline.