Figure 2.

Circulating resistin is required for diet-induced hepatic insulin resistance. (A) Schematic representation of the insulin-clamp procedure. The infusion studies lasted a total of 90 minutes. Mice received a primed-constant infusion of HPLC-purified [3H-3]-glucose (0.1 μCi/min) and insulin (3.6 mU/kg/min) at t = 0 minutes for the duration of the study. A variable infusion of a 10% glucose solution was started and periodically adjusted (glucose as needed) to maintain the plasma glucose concentration at approximately 8 mM for the rest of the study. (B) Rates of glucose uptake (Rd) during the insulin-clamp studies. (C) Rates of endogenous glucose production (GP) during the insulin-clamp studies. (D) Effect of resistin ASO and resistin infusion on total glucose output (in vivo flux-through G6Pase). P < 0.05 vs. SC group; *P < 0.01 vs. SC group; #P < 0.01 vs. HF + ConASO; ##P < 0.01 vs. HF + RsASO.