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. 2015 Jun 15;5(1):10–24.

Figure 3.

Figure 3

Interaction and dependency between AF4 and transcription-associated factors. A. Western-Blot experiment for CDK9, CDK9-T186A, CYCT1, NFkB, AF4, CDK2, CDK7 and MEN1 from whole cell lysates of non-transfected HeLa (left) compared to AF4kd V100 cells (right). Relative quantification was carried out by using the Image Lab 3.0 Software (Bio-Rad). Molecular weight of all proteins are indicated. B. Western-Blot experiments with affinity purified (AP) strep-tagged AF4 (left) and AF4N (right) probed with the same antibodies. C. Immunoprecipitation of BFP-CYCT1 from nuclear fractions of transiently transfected 293T cells. (mock, pETP-BFPCYCT1, pEZP-AF4ST or pEZP-AF4NST; input 0.2%, eluate 33%). BFP-CYCT1 (112 kDa) as well as endogenous CYCT1 (87 kDa) were precipitated. Lower panel: Western blot with antiserum against the AF4 N-terminus.