Gene	Primer Sequence	Reference
Sox17β	F-AACTCCCACCAGCAGGCTACTTTG	(Myers et al., 2004)
	R-TGTCAATGTCACTCTCCAGATGTCC
Mixer	F-CACCAGCCCAGCACTTAACC	(Henry and Melton, 1998)
	R-CAATGTCACATCAACTGAAG
VegT	F-CAAGTAAATGTGAGAAACCGTG	(Zhang et al., 1998)
	R-CAAATACACACACATTTCCCGA
Xbra	F-GGATCGTTATCACCTCTG	(Wilson and Melton, 1994)
	R-GTGTAGTCTGTAGCAGCA
ODC	F-CAGCTAGCTGTGGTGTGG	(Agius et al., 2000)
	R-CAACATGGAAACTCACACC
Cerberus	F-GCTTGCAAAACCTTGCCCTT	(Bouwmeester et al., 1996)
	R-GTGATGGAACAGAGATCTTG
Xhex	F-AACAGCGCATCTAATGGGAC	(Newman et al., 1997)
	R-CCTTTCCGCTTGTGCAGAGG
Mix.1	F-GCAGATGCCAGTTCAGCCAAT	(Wilson and Melton, 1994)
	R-TTTGTCCATAGGTTCCGCCCTG
Bmp2	F-AGCCAGCCGAGCAAATACAGTG
	R-GGTGTCCAATAGTCTCACAACAGGG
Bmp4	F-AGCCCCGTTGTCATAGAAACTTC
	R-AGTGTGCGTGTGGTGATTCCAG
Bmp7	F-TTTATCCAGAGGAGGTTGCGAGGC
	R-TGTCAGCGTCATTGAGGAAGTTG
Alk3	F-TTCTCCAAAAGCCCTGTCACGG
	R-CAATAATGGTAGCCCTGAGCCAC
Xhox-3	F-ATATGATGAGCCACGCAGCAG	(Suzuki et al., 1997)
	R-CAGATGCTGCAGCTCTTTGGC
Msx-1	F-GCTAAAAATGGCTGCTAA
	R-AGG TGG GCT GTG TAA AGT