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. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: Chemistry. 2012 Nov 7;18(51):16349–16357. doi: 10.1002/chem.201202072

Figure 1.

Figure 1

Time dependent absorbance changes upon photolysis at 4 K and thermal cycling of CO-inhibited α-H195Q N2ase in D2O-based 25 mM HEPES, pH 7.4 buffer with 40% ethylene glycol. Left: ‘hi-12C16O’ conditions. Top to bottom: 1st photolysis, time points shown are 0.5, 4, 9, 52s, 3, 30, 150 min; 2nd photolysis, after warming to 120 K for 20 min, time points shown are 0.5, 7, 13, 35s, 2, 30min; 3rd photolysis after poising at ~263 K for 16 h, time points shown are 0.5, 5, 13, 30s, 5, 70, 270 min. Right: ‘hi-13C18O’ conditions. Top to bottom: 1st photolysis, time points shown are: 0.5, 2, 10, 31, 60, and 122 min; 2nd photolysis, after warming sample to 120 K for 10 min, time points shown are: 2, 10, 30, and 60 min; 3rd photolysis, after warming sample to 233 K for 8 h, time points shown are: 3, 23, and 63 min.