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Serum antibodies induced by SQ and IN immunization with recombinant P2 (rP2) assayed by flow cytometry with antisera raised to rP2. Flow cytometry was performed with the homologous strain in triplicate and median fluorescence intensity was determined. IN sera were tested at a 1:10 dilution. SQ sera were tested at a 1:100 dilution. Results are expressed as the mean±s.e.m. Student’s t-test was used to test for significance. SQ, subcutaneous; NMS, normal mouse serum; IN, intranasal.
