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. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: Am J Physiol Cell Physiol. 2007 Apr 18;293(1):C477–C485. doi: 10.1152/ajpcell.00075.2007

Fig. 2.

Fig. 2

Effect of hypoxic agents on BeWo markers of hypoxia. A: serum-starved BeWo cells were incubated in 0.2 mM DFO or 0.2 mM cobalt chloride for up to 24 h (n = 4). At the time points noted, samples were extracted and analyzed for transferrin receptor (TfR) expression compared with untreated control samples. B: serum-starved BeWo cells were incubated in 0.2 mM DFO or 0.2 mM cobalt chloride for 24 h. Samples were extracted, analyzed for GLUT1 (n = 6), TfR (n = 4), and β-actin (n = 3) expression by slot immunoblotting, and compared with untreated controls. *P < 0.05 vs. control.