Skip to main content
. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: Pathog Dis. 2014 Feb 3;70(2):176–184. doi: 10.1111/2049-632X.12119

Figure 1.

Figure 1

Characterization of wild type and mutant B. burgdorferi strains. In panel A growth rates were determined by daily triplicate cell counts using dark field microscopy of cultures grown at 37°C in BSK-H medium (no antibiotics). In panel B the production and surface presentation of OspC by each strain was assessed by immunoblotting and proteinase K treatment, respectively. Proteinase K treated or untreated cells were fractionated by SDS-PAGE, immunoblotted and screened with polyclonal anti-OspC (phyletic type A) or anti-FlaB antiserum. Surface presentation was further demonstrated through indirect immunofluorescence assays using polyclonal anti-OspC antiserum (Panel D). All methods are detailed in text.