Skip to main content
. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: Pathog Dis. 2014 Feb 3;70(2):176–184. doi: 10.1111/2049-632X.12119

Figure 3.

Figure 3

Determination of IgG titer to B. burgdorferi and recombinant OspC proteins in mice infected with B. burgdorferi B31 and ospC mutant strains. Serum was harvested from mice infected by needle inoculation (panel A) or tick bite (panel B), serially diluted, and screened against immobilized whole B. burgdorferi B31 cells (white bar), rOspC (gray bar) and rOspCΔC10 (black bar). Individual mouse titers (indicated by triangles) were calculated as the log of the inverse of the dilution corresponding to 1/3 of the ODmax plateau on the sigmoidal curve. Bars denote the geometric mean titer. All methods are detailed in text.