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. Author manuscript; available in PMC: 2015 Jul 9.
Published in final edited form as: Pathog Dis. 2014 Feb 3;70(2):176–184. doi: 10.1111/2049-632X.12119

Figure 4.

Figure 4

The immunoreactive profiles of mice infected with control and mutant strains by tick bite and needle inoculation. In panel A, ticks were microinjected with each strain and then fed on naïve mice to repletion. Serum was collected after 3 weeks, diluted 1:2000, and used to screen immunoblot of B. burgdorferi B31 cell lysate. In panel B, antiserum from mice challenged with 104 spirochetes of each strain was diluted 1:2000 and used to screen immunoblot of B. burgdorferi B31 cell lysate. Representative data are shown. All methods are detailed in text.