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Elastin IHC (elastin=brown, cell nuclei=purple) and Movat's pentachrome stains (collagen-yellow, PGs/GAGs=blue, elastin=black, cell nuclei=purple) for radial sections at three regions: hinge, belly, and coaptation region. In the hinge, the spongiosa elastin structure started thick and gradually decreased in thickness when moving towards the belly, where it was thinnest. Spongiosa elastin increased again in thickness towards the coaptation region. Scale bar=200 μm.
