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Metformin inhibits growth and RTK signaling of ErbB2– overexpressing breast cancer cells in vitro. A, effect of metformin on the proliferation of the selected cell lines. MCF-10A, BT-474, 78617, and SK-BR-3 cells were treated with metformin at indicated concentrations for 6 days, followed by SRB assay. B, Western blot analysis of RTK expression, activation, and signaling in ErbB2–overexpressing breast cancer cells.
