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Gray line: Direct titration of vanillic acid diethylamide (ligand II) in the presence of 10 μm Hsp90 protein. Black line: Competition experiment with titration of vanillic acid diethylamide (ligand II) as a spy ligand in the presence of 10 μm Hsp90 protein and a constant concentration of 7 mm of 2-aminopyrimidine (ligand III).
