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. 2015 Jan 1;4(1):54–64. doi: 10.1089/biores.2014.0053

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© Hervé Pageon et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited

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FIG. 1. — Histology (hematoxylin–eosin–saffron [HES] coloration) and immunostaining of reconstituted skin (n=4) prepared with collagen modified by CEL (B, E, I, M) and CML (C, F, J, N) as compared to control (A, D, H, L). Immunostaining against α6 integrin (D, E, F), laminin 5 (H, I, J), and procollagen I (L, M, N). Surface staining was quantified with picture analysis software: α6 integrin (G), laminin 5 (K), and procollagen I (O) for each sample. Values are expressed in arbitrary units (results of three measurements of different zones of each of four samples used for immunochemistry). Means±SD are reported (**p<0.01 and *p<0.05). Integrin α6 and procollagen I are increased by CEL and CML, while laminin 5 is decreased by CEL as compared to control skin.