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. 2015 Jan 1;4(1):54–64. doi: 10.1089/biores.2014.0053

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© Hervé Pageon et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited

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FIG. 2. — Histology (HES coloration) and immunostaining of reconstituted skin (n=4) prepared with methyglyoxal-modified collagen rich in hydroimidazolone: MG-H1 (B, D, F, H) as compared to control (A, C, E, G), and glycoxidized collagen (pentosidine [PEN] rich) (M, O, Q, S) as compared to control (L, N, P, R). Immunostaining against α6 integrin (C, D, N,O), laminin 5 (E, F, P, Q), and procollagen I (G, H, R, S). Surface staining was evaluated as in Figure 1 for α6 integrin surface (I, T), laminin 5 (J, U), and procollagen I (K, V). Means±SD are reported (*p<0.05). Laminin 5 is significantly decreased by methylglyoxal-modified collagen compared to control.