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. 2015 Jan 1;4(1):54–64. doi: 10.1089/biores.2014.0053

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© Hervé Pageon et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited

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FIG. 3. — Patterns of gene expression measured by qPCR in epidermal (A) and dermal (B) layers of reconstructed skin exposed to collagen modified by CML, CEL, MG-H1, and pentosidine (PEN). mRNA levels were quantified in epidermis (keratinocytes) or dermis (fibroblasts) using quantitative reverse transcriptase–polymerase chain reaction at the end of culture emersion phase. Each point represents the mean value of normalized mRNA quantity (n=2). qPCR was performed in duplicate on two different samples. Data are expressed in arbitrary units as mean±SEM (*p<0.05).