FIG. 3.

Autophagy-protecting ADSCs against oxidative stress and low-nutrient condition. (A) Cell viability was estimated by WST-1 assay after 600 μM H₂O₂ or 2 mM EGTA treatment in 2% FBS medium for 2 h. *p<0.05. (B) ADSCs were cultured on TCPS for 24 h or CS 72 h. After treatment as described in (A) for 2 h, cells were harvested and subjected for Western blots with anti-LC3 antibodies. ADSCs grown on TCPS for 24 h without treatment were defined as the control group (ctrl). Band intensities were quantified and normalized to GAPDH (the internal control). (C) ULk1, Atg13, and Ambra1 mRNA levels were determined by quantitative RT-PCR after treatment as described in (A). *p<0.05.