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. 2004 Jun 3;23(13):2544–2553. doi: 10.1038/sj.emboj.7600248

Figure 6.

Figure 6

Involvement of Crm1 in the hemin-induced nuclear exclusion of Bach1. (A, B) Localization of GFP-GST-B1(417–587) in 293T cells (A) or Bach1 in GM02063 cells (B) in the presence of Crm1 or Crm1K1 was examined. Cells were treated with indicated reagents. (C) Glutathione beads (lane 4) or glutathione beads with GST–Bach1(417–645) (lane 5) were incubated with liver cell extracts and bound proteins were analyzed by immunoblotting analysis using anti-Crm1 antibodies. Fractions of input extracts were also examined (5.7, 1.9, or 0.6 μg in lanes 1–3, respectively).