Table 1. Correlation between phenotypic (LPT) and genotypic (sequencing) amitraz resistance status.

	Larvae that survived LPT a
Sample	RF of strain c	Genotype d	Phenotype
5AM2(4)A2	100	CC/CC	Amitraz Resistant
5AM2(4)A3	100	CC/CC	Amitraz Resistant
5AM3(8)A1	100	CC/CC	Amitraz Resistant
5AM3(8)A3	100	CC/CC	Amitraz Resistant
3AM2(4)A3	100	CC/CC	Amitraz Resistant
2AM3(16)A3	28	CC/CC	Amitraz Resistant
1AM3(8)A3	10	CC/CC	Amitraz Resistant
	Larvae that did not survive LPTs b
Sample	RF of strain c	Genotype d	Phenotype
2AM3(16)A1	28	AA/TT	Amitraz susceptible
2AM3(16)A2	28	AA/TT	Amitraz susceptible
1AM3(8)A2	10	AA/TT	Amitraz susceptible
1AM3(8)A3	10	AA/TT	Amitraz susceptible
1AM3(8)A1	10	AC/TC	Amitraz susceptible
1AM3(16)A1	10	AC/TC	Amitraz susceptible

Discriminating dose of amitraz used was 250 ppm.

^a Larvae that were alive after the larval packet test assay was completed, meaning they were resistant to the amitraz concentrations applied.

^b Larvae that were susceptible to the concentrations of amitraz used and were dead after the assay was completed.

^c RF is the resistance factor, a RF of 100 implies that the strain is amitraz resistant. RF of 10 and 28 means the strain is susceptible to amitraz.

^d Genotype was inferred based on whether or not the sequenced larvae contained the two SNPs published by Chen, He (26). CC/CC – all four alleles are resistant (homozygous resistant), AA/TT – all four alleles are the wild type alleles (homozygous susceptible), AC/TC – at both SNP positions both the wild type and mutant alleles are present (heterozygous).