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. 2015 Feb 1;4(1):164–174. doi: 10.1089/biores.2014.0057

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© Mayumi Umebayashi et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 2. — GAM transplantation and detection of in vivo gene transfer. (A) Gross appearance of manufactured GAM, just transplanted GAM to cranial bone surface, and transplanted GAM after 2 weeks. (B) GFP expression of migrated cells in the surface area of GAM after 2 weeks of transplantation. Representative images in GAM of BMP4 group are shown. Expression was recognized only in GAM harboring 1 mg of pDNA (GFP, BMP4, and Runx2) groups. DIC, differential interface contrast; GAM, gene-activated matrix. Scale bar is 50 μm.