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. 2015 May 1;4(1):266–277. doi: 10.1089/biores.2015.0004

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© Birgit Weyand et al. 2015; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited.

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FIG. 1. — (a, b) Set up of system. (a) Schematics. 1, Control box; 2, pressure ports; 3, bioreactor vessel with scaffold holder; 4, integrated bypass system; 5, sampling probe (for medium analysis); 6, culture medium reservoir; 7, peristaltic pump; 8, laser light with filters and lenses; 9, photomultiplier (light detector); 10, temperature sensor; 11, OPAL system (optical oxygen measurement system) with built-in sinusoidal frequency generator and lock-in amplifier; 12, computer with software; → medium flow direction. (b) Bioreactor with laser. (c) Setup of static measurement system, the y-shaped light guide is connected to the light source, the light detector, and the OPAL system with the computer software.