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. 2004 Jun 10;23(13):2620–2631. doi: 10.1038/sj.emboj.7600261

Figure 2.

Figure 2

TREX recruitment to four different intronless genes. Single chromatin preparations from yeast strains expressing epitope-tagged Yra1p (KAY136), Sub2p (FSY1473) or Hpr1p (FSY1525) were immunoprecipitated using either antibodies recognizing PolII (8WG16) or the epitope tag. To determine the amount of TREX component recruited to the site of transcription per PolII/nascent RNA complex, the amount of TREX component recruited was divided by the amount of PolII recruited (see Materials and methods for details). The striped bars indicate which primer pairs are located approximately 1000 bp after transcription initiation. (A) Yra1p, (B) Sub2p and (C) Hpr1p. The data presented are the average of two independent experiments.