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. 2004 Jun 10;23(13):2620–2631. doi: 10.1038/sj.emboj.7600261

Figure 4.

Figure 4

Sub2p, Yra1p and Hpr1p are differentially associated with the nascent mRNA. ChIPs with an RNase treatment step were performed on yeast strains expressing epitope-tagged Sub2p (FSY1473), Yra1p (KAY136) and Hpr1p (FSY1525). The methods used are identical to those described in Figure 3. The PMA1 primer pairs used are described in Figure 1. The RNase sensitivity of Sub2p is almost identical to MS-HA; only 20–40% of the Sub2p remains at the site of transcription after RNase treatment (n=4). In contrast, the RNase sensitivity of Hpr1p (n=4) resembles that of PolII (n=10); it is insensitive to RNase treatment. Yra1p shows partial RNase sensitivity (n=7): it is more RNase sensitive than PolII at both the 5′ and middle primer; however this difference is statistically significant only at the 5′ end of PMA1.