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. 2004 Jun 10;23(13):2620–2631. doi: 10.1038/sj.emboj.7600261

Figure 6.

Figure 6

Yra1p and Sub2p are poorly recruited to a subset of intron-containing genes. ChIPs were performed on strains expressing epitope-tagged Yra1p (KAY136), Sub2p (FSY1473), Hpr1p (FSY1525) and the U1 SnRNP component, Prp42p (YKK25). The amount of Yra1p, Sub2p, Hpr1p and U1 snRNP recruited per PolII/nascent RNA complex is examined on five different intron-containing genes. The primer pairs used are described in Figure 5A and the striped bars (A–C) indicate primer pairs that are approximately 1000 bp after transcription initiation and the checkered bars (D) indicate the position of the intron. (A) Hpr1p, (B) Yra1p, (C) Sub2p and (D) U1 snRNP. PCR reactions for all nine genes were performed from the same ChIP samples; therefore, gene-to-gene differences in TREX and U1 snRNP recruitment cannot be attributed to experimental variation. The data presented are the average of two independent experiments.